RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Schoolmaster ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus apodus
FDA Seafood List Common Name: Schoolmaster
FDA Seafood List Market Name: Schoolmaster
Unique IEF Gel Code: 19940829gel01
Unique Web Code: asg01Srr
Tissue Aliquot Code: lutjapod H94-002 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	31	2.7	0.239839	17	6.80255	20.4	16.8	3.5	30	16.8	0.228749
2	4.55	193	16.7	0.278576	6	2.37525	7.1	5.9	4.55	193.1	5.6	0.23863
3	5.2	279	24.1	0.344408	5	2.87729	8.6	7.1	5.2	278.7	4.8	0.334985
4	5.85	420	36.3	0.45554	4	3.2219	9.6	8	5.85	419.8	4	0.458265
5	6.55	599	51.8	0.318386	5	2.85749	8.5	7.1	6.55	598.6	5.4	0.297996
6	6.85	638	55.2	0.130452	7	1.39688	4.2	3.5	6.85	638	6.7	0.1176
7	7.35	728	63	0.285289	5	2.56732	7.7	6.4	7.35	727	5.1	0.285066
8	8.15	874	75.6	0.0959495	10	1.66277	5	4.1	8.15	877.3	9.9	0.0946888
9	8.45	950	82.2	0.141263	7	1.75314	5.2	4.3	8.45	949.2	7.1	0.139054
10	8.65	992	85.8	0.156027	5	1.35673	4.1	3.4	8.65	991.7	5	0.152535
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1139	98.5	0.630276	6	6.5557	19.6	16.2	9.3	1137.7	6.1	0.606752

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 9
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	41	3.5	0.428957	7	5.01546	9.7	8.6	3.5	40.7	6.7	0.420893
2	3.559	50	4.3	0.251514	4	1.403	2.7	2.4	3.559	51.2	3.9	0.205094
3	4.071	128	11.1	0.137873	5	1.12842	2.2	1.9	4.071	128	4.8	0.132758
4	4.301	163	14.1	0.507819	5	4.67085	9	8.1	4.301	163.2	5.1	0.518169
5	4.55	201	17.4	0.175764	7	1.93386	3.7	3.3	4.55	200.7	6.9	0.158602
6	5.2	286	24.7	0.170407	5	1.54008	3	2.7	5.2	286	5.2	0.168511
7	5.265	300	26	0.0907686	3	0.503207	1	0.9	5.265	298.4	3	0.0938719
8	5.298	307	26.6	0.44738	4	2.98407	5.7	5.1	5.298	307.5	3.8	0.442145
9	5.434	336	29.1	0.0680976	6	0.622348	1.2	1.1	5.434	335.5	5.6	0.0622582
10	5.537	358	31	0.0995364	6	0.881931	1.7	1.5	5.537	358.4	6	0.0834158
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.663	385	33.3	0.0986795	6	1.0003	1.9	1.7	5.663	384.4	6.1	0.0928068
12	5.789	412	35.6	0.384273	4	2.97021	5.7	5.1	5.789	413	4.3	0.393037
13	5.85	425	36.8	0.262572	5	2.11533	4.1	3.6	5.85	424.2	4.7	0.255522
14	5.912	440	38.1	0.317392	4	2.43847	4.7	4.2	5.912	440.1	4.3	0.317352
15	6.012	464	40.1	0.121466	7	1.43344	2.8	2.5	6.012	463	6.8	0.118954
16	6.065	477	41.3	0.032477	3	0.174185	0.3	0.3	6.065	475.9	2.8	0.0347029
17	6.281	529	45.8	0.0597634	4	0.395077	0.8	0.7	6.281	528.9	4.2	0.0529516
18	6.339	543	47	0.189357	7	2.27383	4.4	3.9	6.339	542.8	6.9	0.184677
19	6.434	566	49	0.194483	4	1.48159	2.9	2.6	6.434	566.2	4.5	0.186608
20	6.55	594	51.4	0.132068	6	1.29782	2.5	2.2	6.55	592.6	5.9	0.12315
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.667	610	52.8	0.0779832	3	0.371515	0.7	0.6	6.667	610.2	2.7	0.0762918
22	6.762	623	53.9	0.191973	2	0.567597	1.1	1	6.762	622.6	1.7	0.19068
23	6.85	635	54.9	0.0914024	6	0.846183	1.6	1.5	6.85	635.8	5.7	0.0834808
24	7.097	679	58.7	0.289964	3	1.40403	2.7	2.4	7.097	678.7	2.7	0.298795
25	7.142	687	59.4	0.0798127	3	0.437544	0.8	0.8	7.142	686.4	3.4	0.0727498
26	7.35	724	62.6	0.264252	5	2.20001	4.2	3.8	7.35	723.3	4.7	0.265195
27	8.15	872	75.4	0.186778	5	1.65354	3.2	2.9	8.15	873.5	5.4	0.173065
28	8.45	945	81.7	0.112448	9	1.6824	3.2	2.9	8.45	945.6	8.5	0.111399
29	8.65	993	85.9	0.147805	5	1.31984	2.5	2.3	8.65	991.9	5.4	0.137971
30	9.3	1136	98.3	0.568565	5	5.2262	10.1	9	9.3	1135.4	5.2	0.570638

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.52	39	3.4	0.425957	6	4.47436	12.7	9.8	3.52	38.9	5.8	0.43198
2	3.579	48	4.2	0.294529	4	1.81509	5.1	4	3.579	48.7	3.9	0.259423
3	4.086	125	10.8	0.141847	5	1.14616	3.2	2.5	4.086	125.5	4.7	0.136881
4	4.323	161	13.9	0.515559	5	4.81786	13.6	10.6	4.323	160.9	5.1	0.529227
5	5.239	288	24.9	0.0517739	6	0.577763	1.6	1.3	5.239	288.1	5.8	0.0558267
6	5.28	297	25.7	0.123331	3	0.540311	1.5	1.2	5.28	296.1	2.6	0.116785
7	5.317	305	26.4	0.480249	4	3.34645	9.5	7.3	5.317	305	4	0.47746
8	5.45	334	28.9	0.0634496	5	0.496971	1.4	1.1	5.45	334	4.7	0.0590978
9	5.551	356	30.8	0.098895	5	0.795213	2.2	1.7	5.551	357	5.3	0.0850809
10	5.675	383	33.1	0.0975659	5	0.864735	2.4	1.9	5.675	382.6	5.3	0.0912021
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.804	411	35.6	0.410015	3	2.08161	5.9	4.6	5.804	411.1	3.3	0.357565
12	5.858	423	36.6	0.113712	9	2.12598	6	4.7	5.858	418.7	8.7	0.138059
13	5.922	439	38	0.341828	4	2.47805	7	5.4	5.922	438.4	4.1	0.339836
14	6.015	462	40	0.136233	6	1.51273	4.3	3.3	6.015	460.7	6.4	0.133754
15	6.067	475	41.1	0.0287017	3	0.177429	0.5	0.4	6.067	473.6	2.7	0.0367636
16	6.268	525	45.4	0.105957	3	0.323928	0.9	0.7	6.268	524.9	2.5	0.0729248
17	6.317	537	46.5	0.106439	13	2.22279	6.3	4.9	6.317	538.6	13.2	0.0952837
18	6.421	563	48.7	0.295549	5	2.37806	6.7	5.2	6.421	563.4	4.8	0.280846
19	6.522	588	50.9	0.0500665	3	0.298537	0.8	0.7	6.522	588.4	3.5	0.0487326
20	6.623	605	52.3	0.0675972	7	0.590875	1.7	1.3	6.623	603.2	6.7	0.0494815
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.711	617	53.4	0.151446	2	0.437216	1.2	1	6.711	617.3	1.6	0.151806
22	7.051	672	58.1	0.318376	2	1.41308	4	3.1	7.051	672.5	2.5	0.32269
23	7.101	681	58.9	0.0630183	4	0.442929	1.3	1	7.101	679.4	4	0.0622573

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	31	2.7	0.24397	13	5.464	15.8	12.6	3.5	29.1	12.6	0.244004
2	4.55	190	16.4	0.247745	6	2.26296	6.5	5.2	4.55	189.5	6	0.213925
3	5.2	273	23.6	0.337078	5	2.82681	8.2	6.5	5.2	273.3	4.9	0.325886
4	5.85	417	36.1	0.435335	4	3.04344	8.8	7	5.85	416.6	4	0.431556
5	6.55	596	51.6	0.301145	5	2.71664	7.8	6.3	6.55	595.5	5.4	0.285067
6	6.85	637	55.1	0.12033	7	1.22638	3.5	2.8	6.85	636.5	6.5	0.105951
7	7.35	727	62.9	0.269805	5	2.41138	7	5.6	7.35	727	5.1	0.268228
8	8.15	879	76	0.105698	10	1.86021	5.4	4.3	8.15	880.4	10.2	0.102771
9	8.45	952	82.4	0.152016	6	1.67744	4.8	3.9	8.45	951.4	6.3	0.149918
10	8.65	994	86	0.154889	5	1.36104	3.9	3.1	8.65	994.2	5	0.153333
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1134	98.1	0.954324	6	9.80064	28.3	22.6	9.3	1135.1	5.8	0.956319

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.559	40	3.5	0.444065	9	7.12166	18.7	15.1	3.559	42	9.3	0.43086
2	4.102	123	10.6	0.153611	5	1.21089	3.2	2.6	4.102	122.7	4.6	0.149105
3	4.324	157	13.6	0.518964	5	4.61555	12.2	9.8	4.324	156.7	4.8	0.54069
4	5.218	282	24.4	0.0686747	6	0.711207	1.9	1.5	5.218	283.5	6.1	0.0661177
5	5.267	293	25.3	0.137196	2	0.553316	1.5	1.2	5.267	292	2.1	0.147687
6	5.303	301	26	0.482754	4	3.35501	8.8	7.1	5.303	300.9	3.8	0.502311
7	5.442	332	28.7	0.0714961	5	0.504303	1.3	1.1	5.442	331.5	4.5	0.0626736
8	5.541	354	30.6	0.0929649	4	0.593829	1.6	1.3	5.541	355	3.7	0.0912847
9	5.671	383	33.1	0.106203	7	1.11017	2.9	2.4	5.671	382	7.2	0.0865288
10	5.796	411	35.5	0.385731	4	2.22065	5.8	4.7	5.796	410.9	3.6	0.34535
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.841	421	36.4	0.168328	7	2.31635	6.1	4.9	5.841	418.6	6.9	0.189153
12	5.913	439	37.9	0.335932	5	2.7615	7.3	5.8	5.913	438.3	4.7	0.329979
13	6.003	462	39.9	0.13656	7	1.53914	4.1	3.3	6.003	461.6	6.8	0.127696
14	6.046	473	40.9	0.0400316	3	0.240168	0.6	0.5	6.046	474.4	3.4	0.0396964
15	6.258	527	45.5	0.112639	3	0.554374	1.5	1.2	6.258	526.2	3.4	0.0916888
16	6.301	538	46.5	0.121734	10	2.05178	5.4	4.3	6.301	540.1	9.8	0.118016
17	6.415	567	49	0.327414	4	2.36608	6.2	5	6.415	566.9	4.1	0.322925
18	6.501	589	50.9	0.0366269	3	0.283605	0.7	0.6	6.501	591.2	3.3	0.0487871
19	6.548	601	51.9	0.0586489	5	0.46767	1.2	1	6.548	601.7	4.8	0.0552772
20	6.605	609	52.6	0.117677	3	0.487135	1.3	1	6.605	608.8	2.6	0.10767
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.693	621	53.7	0.217449	2	0.692458	1.8	1.5	6.693	620.6	1.8	0.218983
22	7.031	675	58.3	0.359822	3	1.64888	4.3	3.5	7.031	675.1	2.6	0.353386
23	7.075	683	59	0.082273	4	0.577715	1.5	1.2	7.075	682.8	4.4	0.0733889

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	29	2.5	0.216603	15	5.67621	18.8	15.4	3.5	28.4	14.7	0.218392
2	4.55	191	16.5	0.225887	7	2.31257	7.6	6.3	4.55	191.1	6.6	0.199121
3	5.2	281	24.3	0.29264	6	2.8805	9.5	7.8	5.2	281	5.7	0.284471
4	5.85	427	36.9	0.294491	7	3.77174	12.5	10.2	5.85	426.7	7.2	0.29395
5	6.55	605	52.3	0.270655	6	2.79305	9.2	7.6	6.55	603.8	6.3	0.250385
6	6.85	646	55.9	0.121151	7	1.35642	4.5	3.7	6.85	646.2	7	0.109521
7	7.35	736	63.7	0.262804	6	2.53558	8.4	6.9	7.35	735.8	5.5	0.259992
8	8.15	891	77.1	0.0710848	10	1.22402	4	3.3	8.15	891.3	10.1	0.0684912
9	8.45	962	83.2	0.108685	7	1.29398	4.3	3.5	8.45	961.4	6.8	0.107144
10	8.65	1003	86.8	0.111302	6	1.10392	3.6	3	8.65	1003.3	5.7	0.11018
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1135	98.2	0.415636	10	5.32136	17.6	14.4	9.3	1133.4	9.7	0.308654

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK CO SP MK SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 109

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 20.400 4.550 7.100 5.200 8.600 5.850 9.600 6.550 8.500 6.850 4.200 7.350 7.700 8.150 5.000 8.450 5.200 8.650 4.100 9.300 19.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 8 LANE TYPE: pI Markers ACTUAL GEL LANE: 10 PeakCount: 11 Return to Lane Navigator
3.500 9.700 3.559 2.700 4.071 2.200 4.301 9.000 4.550 3.700 5.200 3.000 5.265 1.000 5.298 5.700 5.434 1.200 5.537 1.700 5.663 1.900 5.789 5.700 5.850 4.100 5.912 4.700 6.012 2.800 6.065 0.300 6.281 0.800 6.339 4.400 6.434 2.900 6.550 2.500 6.667 0.700 6.762 1.100 6.850 1.600 7.097 2.700 7.142 0.800 7.350 4.200 8.150 3.200 8.450 3.200 8.650 2.500 9.300 10.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 9 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 11 PeakCount: 30 Return to Lane Navigator
3.520 12.700 3.579 5.100 4.086 3.200 4.323 13.600 5.239 1.600 5.280 1.500 5.317 9.500 5.450 1.400 5.551 2.200 5.675 2.400 5.804 5.900 5.858 6.000 5.922 7.000 6.015 4.300 6.067 0.500 6.268 0.900 6.317 6.300 6.421 6.700 6.522 0.800 6.623 1.700 6.711 1.200 7.051 4.000 7.101 1.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 10 LANE TYPE: Specimen ACTUAL GEL LANE: 12 PeakCount: 23 Return to Lane Navigator
3.500 15.800 4.550 6.500 5.200 8.200 5.850 8.800 6.550 7.800 6.850 3.500 7.350 7.000 8.150 5.400 8.450 4.800 8.650 3.900 9.300 28.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator
3.559 18.700 4.102 3.200 4.324 12.200 5.218 1.900 5.267 1.500 5.303 8.800 5.442 1.300 5.541 1.600 5.671 2.900 5.796 5.800 5.841 6.100 5.913 7.300 6.003 4.100 6.046 0.600 6.258 1.500 6.301 5.400 6.415 6.200 6.501 0.700 6.548 1.200 6.605 1.300 6.693 1.800 7.031 4.300 7.075 1.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 23 Return to Lane Navigator
3.500 18.800 4.550 7.600 5.200 9.500 5.850 12.500 6.550 9.200 6.850 4.500 7.350 8.400 8.150 4.000 8.450 4.300 8.650 3.600 9.300 17.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Schoolmaster ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Schoolmaster ---- Go to Display Gel Lane Navigator.