RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pollock ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pollachius virens
FDA Seafood List Common Name: Pollock
FDA Seafood List Market Name: Pollock
Unique IEF Gel Code: 19930823gel12
Unique Web Code: aph02Srr
Tissue Aliquot Code: pollvire 001 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 1
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 1
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	37	4.6	0.0323319	5	0.270876	4.5	3.5	4.55	38.1	4.8	0.0317897
2	5.2	135	17	0.0736502	4	0.536062	8.8	6.8	5.2	135.2	4.5	0.0679112
3	5.85	252	31.7	0.141559	5	1.13613	18.7	14.5	5.85	251.9	4.7	0.137067
4	6.55	386	48.5	0.106301	6	1.00044	16.4	12.8	6.55	385.3	5.7	0.0988385
5	6.85	415	52.1	0.0149698	5	0.173122	2.8	2.2	6.85	417.4	5.1	0.0192769
6	7.35	482	60.6	0.0377164	5	0.316309	5.2	4	7.35	482.7	4.9	0.0366758
7	8.15	603	75.8	0.0708264	7	0.793696	13	10.1	8.15	602.8	6.5	0.0688762
8	8.45	661	83	0.0553628	7	0.709691	11.7	9.1	8.45	661	7.3	0.0545648
9	8.65	713	89.6	0.0333238	6	0.32868	5.4	4.2	8.65	713.1	5.9	0.0315005
10	9.3	768	96.5	0.0650506	8	0.819227	13.5	10.5	9.3	767.5	8.5	0.0545077

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	37	4.6	0.0221658	3	0.0641027	0.3	0.2	4.55	36.6	2.6	0.0137513
2	4.718	62	7.8	0.175145	13	3.92286	18.3	15.1	4.718	61.9	13.4	0.165486
3	4.992	103	12.9	0.0885667	12	1.83871	8.6	7.1	4.992	104.1	12.1	0.0860155
4	5.2	134	16.8	0.0300326	3	0.145117	0.7	0.6	5.2	133.8	3.1	0.0267911
5	5.284	149	18.7	0.228544	4	1.55132	7.3	6	5.284	149.2	3.9	0.225994
6	5.413	172	21.6	0.127246	4	0.819654	3.8	3.2	5.413	171.6	3.7	0.126527
7	5.85	250	31.4	0.0928324	4	0.692344	3.2	2.7	5.85	250.3	4.3	0.0916609
8	5.979	275	34.5	0.53139	3	3.19083	14.9	12.3	5.979	275	3.5	0.519141
9	6.066	292	36.6	0.335031	4	2.34176	10.9	9	6.066	291.7	4.2	0.318062
10	6.169	312	39.1	0.193988	3	0.974737	4.6	3.8	6.169	311.9	3	0.183098
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.231	324	40.7	0.064853	4	0.453276	2.1	1.7	6.231	324.2	4	0.0632035
12	6.277	333	41.8	0.0584144	3	0.286719	1.3	1.1	6.277	333.2	2.8	0.0569898
13	6.344	346	43.4	0.0194145	2	0.0766946	0.4	0.3	6.344	345.8	2.2	0.0199232
14	6.437	364	45.7	0.155925	5	1.33239	6.2	5.1	6.437	362.6	5.4	0.140227
15	6.514	379	47.6	0.0354175	7	0.478317	2.2	1.8	6.514	377.5	7.1	0.0382613
16	6.85	414	51.9	0.0160834	5	0.150122	0.7	0.6	6.85	415.2	5.5	0.0154909
17	7.35	487	61.1	0.0391595	5	0.366236	1.7	1.4	7.35	487.1	5.5	0.0376217
18	8.15	607	76.2	0.0589542	4	0.371264	1.7	1.4	8.15	606.3	4	0.0523735
19	8.45	668	83.8	0.0343779	7	0.395381	1.8	1.5	8.45	668.9	7	0.0319523
20	8.65	719	90.2	0.0416589	11	0.713604	3.3	2.8	8.65	721.1	11.1	0.0363211
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	9.03	750	94.1	0.0533325	13	1.21354	5.7	4.7	9.03	750.9	13.1	0.0521931
22	9.3	772	96.9	0.0184207	1	0.0081953	0	0	9.3	772	1.2	0.0039942

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.622	51	6.4	0.243606	7	2.11684	6.4	5	4.622	49.5	6.7	0.17757
2	4.738	68	8.5	0.211102	16	5.80865	17.5	13.7	4.738	66.1	16.3	0.201301
3	5.012	108	13.5	0.154438	14	3.95907	11.9	9.4	5.012	107.3	14.4	0.155342
4	5.312	155	19.4	0.40504	4	2.62645	7.9	6.2	5.312	154.8	3.7	0.399814
5	5.444	178	22.3	0.23676	4	1.46179	4.4	3.5	5.444	178	3.5	0.234019
6	5.863	251	31.4	0.034669	7	0.38407	1.2	0.9	5.863	251.3	6.9	0.0315284
7	5.984	275	34.4	0.639597	4	4.14317	12.5	9.8	5.984	275.7	3.8	0.622157
8	6.07	292	36.5	0.458429	5	3.65557	11	8.7	6.07	292	4.8	0.432861
9	6.166	311	38.9	0.332913	2	1.34069	4	3.2	6.166	311.3	2.4	0.316118
10	6.232	324	40.6	0.105912	3	0.5678	1.7	1.3	6.232	324	3	0.105796
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.277	333	41.7	0.113807	2	0.472358	1.4	1.1	6.277	332.7	2.4	0.113151
12	6.348	347	43.4	0.0321957	3	0.142326	0.4	0.3	6.348	346.5	2.7	0.0296626
13	6.439	365	45.7	0.267388	5	1.82103	5.5	4.3	6.439	364	4.6	0.222742
14	8.674	718	89.9	0.0796116	16	2.24346	6.8	5.3	8.674	723.8	16.5	0.0768418
15	9.1	753	94.2	0.120021	11	2.46611	7.4	5.8	9.1	750.5	11.5	0.121106

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	44	5.5	0.0687566	6	0.631535	7.9	6.3	4.55	43.9	5.6	0.0641383
2	5.2	137	17.1	0.0930892	5	0.818246	10.3	8.1	5.2	136.7	5.2	0.0892692
3	5.85	247	30.8	0.150583	5	1.34775	16.9	13.3	5.85	247.5	5.1	0.148531
4	6.55	388	48.4	0.130172	5	1.21907	15.3	12.1	6.55	386.9	5.5	0.125624
5	6.85	417	52.1	0.0223742	5	0.182411	2.3	1.8	6.85	416.5	4.9	0.021198
6	7.35	480	59.9	0.060736	4	0.459719	5.8	4.6	7.35	480.6	4.4	0.0592643
7	8.15	606	75.7	0.0936103	6	0.964307	12.1	9.5	8.15	605.7	6	0.0906372
8	8.45	661	82.5	0.0777237	7	0.930643	11.7	9.2	8.45	659.8	6.8	0.0768717
9	8.65	713	89	0.0515758	5	0.417611	5.2	4.1	8.65	712.5	4.7	0.0500902
10	9.3	767	95.8	0.0774699	8	0.984292	12.4	9.7	9.3	766.6	8.2	0.0677289

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.569	45	5.6	0.108815	7	1.45954	5.7	4.3	4.569	48.6	7.2	0.114543
2	4.711	67	8.3	0.14397	14	3.54482	13.8	10.3	4.711	65.5	14.1	0.141409
3	5.02	115	14.3	0.0370744	8	0.5437	2.1	1.6	5.02	114.4	8.3	0.0369431
4	5.2	143	17.8	0.0024232	1	0	0	0	5.2	133	1	0
5	5.324	163	20.3	0.143801	9	2.07389	8.1	6.1	5.324	159.4	8.5	0.137367
6	5.392	174	21.7	0.0825527	7	1.08167	4.2	3.2	5.392	178.2	7.4	0.0829347
7	5.85	248	30.9	0.0642553	5	0.604729	2.4	1.8	5.85	249	5.5	0.0625757
8	6.012	280	34.9	0.389358	5	3.6921	14.4	10.8	6.012	279.4	5.3	0.391558
9	6.098	297	37	0.329993	5	2.76011	10.8	8.1	6.098	297.6	4.9	0.317012
10	6.209	319	39.7	0.247241	3	1.18292	4.6	3.5	6.209	318.2	2.8	0.2412
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.265	330	41.1	0.0992242	3	0.607196	2.4	1.8	6.265	330.3	3.4	0.0995698
12	6.31	339	42.2	0.0903176	2	0.379009	1.5	1.1	6.31	338.6	2.3	0.091324
13	6.371	351	43.7	0.0288564	2	0.111235	0.4	0.3	6.371	350.9	2.3	0.0276366
14	6.452	367	45.7	0.234751	4	1.63443	6.4	4.8	6.452	366.5	4.5	0.20666
15	6.523	381	47.4	0.0108683	2	0.0296631	0.1	0.1	6.523	381	1.6	0.0107326
16	6.85	414	51.6	0.0178922	5	0.160122	0.6	0.5	6.85	414.7	5.2	0.0172352
17	7.35	477	59.4	0.0533691	5	0.467896	1.8	1.4	7.35	477.4	5.1	0.0518075
18	8.45	660	82.2	0.0133469	6	0.139402	0.5	0.4	8.45	661.2	5.8	0.0135388
19	8.65	715	89	0.0857022	13	1.75107	6.8	5.1	8.65	717.7	13.2	0.0746062
20	9.083	751	93.5	0.138779	14	3.28346	12.8	9.6	9.083	747.3	13.7	0.135643
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	9.3	769	95.8	0.0313279	4	0.139232	0.5	0.4	9.3	770.2	4.2	0.0184957

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.627	51	6.3	0.21014	13	2.84813	7.7	5.6	4.627	48.6	12.8	0.125984
2	4.71	64	8	0.231372	22	7.49094	20.2	14.7	4.71	70.5	22.5	0.187844
3	5.107	126	15.7	0.133142	19	4.22293	11.4	8.3	5.107	120.5	18.6	0.128344
4	5.368	168	20.9	0.458211	3	2.528	6.8	5	5.368	167.9	3.3	0.436026
5	5.478	186	23.1	0.282821	3	1.49061	4	2.9	5.478	185.9	3	0.283605
6	5.881	253	31.5	0.0265248	7	0.326652	0.9	0.6	5.881	253.8	6.9	0.0265606
7	5.993	275	34.2	0.587057	4	3.7878	10.2	7.4	5.993	275.7	3.8	0.567639
8	6.075	291	36.2	0.404977	6	3.67741	9.9	7.2	6.075	291.8	5.6	0.369204
9	6.178	311	38.7	0.224426	3	1.375	3.7	2.7	6.178	310.2	3.4	0.231277
10	6.239	323	40.2	0.0738409	3	0.435932	1.2	0.9	6.239	323.1	3.2	0.0761285
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.28	331	41.2	0.0778504	3	0.501456	1.3	1	6.28	331.1	3.5	0.0814567
12	6.357	346	43	0.023656	3	0.129413	0.3	0.3	6.357	345.3	2.9	0.0249947
13	6.449	364	45.3	0.217083	5	1.82294	4.9	3.6	6.449	362.5	5.2	0.199119
14	6.575	386	48	0.022355	4	0.150255	0.4	0.3	6.575	387	4.5	0.01899
15	8.715	719	89.4	0.0949207	23	4.42764	11.9	8.7	8.715	727	22.6	0.110372
16	9.01	744	92.5	0.156154	10	1.9422	5.2	3.8	9.01	748	10.1	0.108042

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	38	4.7	0.0815011	6	0.766548	8.2	6.6	4.55	38.8	5.7	0.0752636
2	5.2	140	17.3	0.0954643	5	0.852712	9.1	7.4	5.2	140.5	5.3	0.0906103
3	5.85	248	30.7	0.189831	4	1.4483	15.5	12.5	5.85	247.8	4.4	0.184849
4	6.55	383	47.5	0.168825	5	1.33042	14.2	11.5	6.55	382.5	4.7	0.159471
5	6.85	411	50.9	0.0187001	5	0.175402	1.9	1.5	6.85	410.7	5.4	0.0183846
6	7.35	479	59.4	0.0806839	4	0.620355	6.6	5.3	7.35	479.3	4.5	0.0779084
7	8.15	604	74.8	0.0873649	8	1.12968	12.1	9.7	8.15	604.6	8	0.0799173
8	8.45	660	81.8	0.0902192	9	1.4423	15.4	12.4	8.45	659.8	8.9	0.0914765
9	8.65	714	88.5	0.0706763	4	0.53013	5.7	4.6	8.65	713.9	4.4	0.068146
10	9.3	770	95.4	0.0941805	7	1.05235	11.3	9.1	9.3	769.5	6.7	0.0888639

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 104

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 4.500 5.200 8.800 5.850 18.700 6.550 16.400 6.850 2.800 7.350 5.200 8.150 13.000 8.450 11.700 8.650 5.400 9.300 13.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 1 PeakCount: 10 Return to Lane Navigator
4.550 0.300 4.718 18.300 4.992 8.600 5.200 0.700 5.284 7.300 5.413 3.800 5.850 3.200 5.979 14.900 6.066 10.900 6.169 4.600 6.231 2.100 6.277 1.300 6.344 0.400 6.437 6.200 6.514 2.200 6.850 0.700 7.350 1.700 8.150 1.700 8.450 1.800 8.650 3.300 9.030 5.700 9.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 2 PeakCount: 22 Return to Lane Navigator
4.622 6.400 4.738 17.500 5.012 11.900 5.312 7.900 5.444 4.400 5.863 1.200 5.984 12.500 6.070 11.000 6.166 4.000 6.232 1.700 6.277 1.400 6.348 0.400 6.439 5.500 8.674 6.800 9.100 7.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 15 Return to Lane Navigator
4.550 7.900 5.200 10.300 5.850 16.900 6.550 15.300 6.850 2.300 7.350 5.800 8.150 12.100 8.450 11.700 8.650 5.200 9.300 12.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.569 5.700 4.711 13.800 5.020 2.100 5.200 0 5.324 8.100 5.392 4.200 5.850 2.400 6.012 14.400 6.098 10.800 6.209 4.600 6.265 2.400 6.310 1.500 6.371 0.400 6.452 6.400 6.523 0.100 6.850 0.600 7.350 1.800 8.450 0.500 8.650 6.800 9.083 12.800 9.300 0.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 21 Return to Lane Navigator
4.627 7.700 4.710 20.200 5.107 11.400 5.368 6.800 5.478 4.000 5.881 0.900 5.993 10.200 6.075 9.900 6.178 3.700 6.239 1.200 6.280 1.300 6.357 0.300 6.449 4.900 6.575 0.400 8.715 11.900 9.010 5.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 16 Return to Lane Navigator
4.550 8.200 5.200 9.100 5.850 15.500 6.550 14.200 6.850 1.900 7.350 6.600 8.150 12.100 8.450 15.400 8.650 5.700 9.300 11.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pollock ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pollock ---- Go to Display Gel Lane Navigator.