Contacts

Joseph S. Wall James F. Hainfeld Martha N. Simon Frank E. Kito Beth Yu Lin

wall@bnl.gov hainfeld@bnl.gov msimon@bnl.gov fkito@bnl.gov bylin@bnl.gov

tel: (631) 344-2912 tel: (631) 344-3367 tel: (631) 344-3372 tel: (631) 344-3372 tel: (631) 344-3372

Biology Department, Bldg 463 Brookhaven National Lab Upton, NY 11973-5000 tel: (631) 344-3372 fax: (631) 344-3407

Facility

STEM is a custom-built electron microscope optimized for imaging unstained biological molecules with minimal radiation damage. The group at Brookhaven operates STEM as a User Facility with partial DOE and partial Fee-for-Service support.

Applications

STEM is a quantitative microscopy, and at BNL it is optimized for structure determination of biological molecules, complexes, and organelles. Freeze-dried, unstained, unshadowed samples are imaged in-focus which permits: 1.   Mass measurements to:   -   Determine stoichiometry and homogeneity of a complex.   -   Compare modified or re-assembled complexes with native structures.   -   Determine filament symmetry from mass per unit length. 2.   Optimal visualization of metal clusters used as specific heavy atom labels. 3.   Simultaneous imaging of DNA, protein, and gold labels in a complex.

Video

The role of the STEM and cryo-EM in structural biology is summarized by Joe Wall in this 3:30 minute STEM Video clip viewable with Real Player.

Updated 17 Dec 2008	Security Notice   Webteam   Site Map   STEM   Biology   BNL