![](https://webarchive.library.unt.edu/eot2008/20080916212157im_/http://newton.dep.anl.gov/askasci/images/New_main/corner-l.gif) |
DNA Pellets
|
![](https://webarchive.library.unt.edu/eot2008/20080916212157im_/http://newton.dep.anl.gov/askasci/images/New_main/corner-R.gif) |
|
Welcome Teachers and Students
Visit
Our Archives
How to
Ask a Question
Ask
A Question
Question
of the Week
Our
Expert Scientists
About
Ask A Scientist
Referencing
NEWTON BBS Articles
Frequently Asked Questions |
DNA Pellets
Name: Donne
Status: student
Grade: 9-12
Location: N/A
Question: What is a DNA pellet? How can one define this term
logically and scientifically?
---------------------------------------
This refers to the mass of DNA at the bottom of the centrifuge tube after
you do a DNA extraction. DNA is insoluble in ethanol, so when you add
ethanol to a mixture containing DNA, the DNA will precipitate in the
ethanol. Centrifuging will leave a 'pellet' of DNA.
Hope this helps,
Burr
====================================================================
I am assuming you are extracting DNA and it wants you to pellet the DNA.
One molecule of DNA is too small to see with the even the most powerful
microscope. In order to see it you need millions of strands wrapped
together. If you then spin this in a centrifuge it will form a visible mass
in the bottom of the tube. It will appear as a white dot in the bottom of
the tube, also known as a pellet.
Vanhoeck
====================================================================
A DNA pellet is a clump of DNA that has been precipitated from an aquaeous
solution by the addition of alcohol.
Ron Baker, Ph.D.
====================================================================
|
|
We provide a means to have questions answered that are not going to be easily found on the web or within common references.
Return to NEWTON's HOME PAGE
For
assistance with NEWTON contact a System Operator, at Argonne's Division
of Educational Programs
NEWTON
BBS AND ASK A SCIENTIST Division of Educational Programs
Building
DEP/223 9700 S. Cass Ave. Argonne,
Illinois 60439-4845
USA
Last
Update:
July 2008
|