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SAGE

Human SAGE Genie Tools

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Digital Karyotyping

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SAGE Genie DGED set-up for two SAGE pools

What the SAGE DGED Tool Can Do

The SAGE Digital Gene Expression Displayer is a tool that identifies those genes that are expressed at significantly different levels (as defined by the user) in two pools of human libraries, based on SAGE tag analysis. The algorithm takes into account the differences in sample size between Pools A and B, which can be large. The user selects a value for statistical significance (P value) and a value for the difference in the level of expression (F value) between the two pools. The results are based on the sequence odds ratio and measure of significance.

Need help? If this is your first time using the DGED and you need help and examples, please visit All About the Digital Gene Expression Display Tool.

Use the SAGE Digital Gene Expression Displayer

Note: SAGE tags are either:
        • "short" (10 bp), including "extracted short" (10 bp extracted from 17bp tag) or
        • "long" (17 bp).

1. Select: Short tags or Long tags
2. List libraries by:
3. For Pool A, select library criteria or enter the name of a library. Repeat for Pool B.

Pool A Pool B
Tissue Type:


Include
Exclude
Tissue Histology:
Exclude Cell Lines:
Library Name:
Tissue Type:


Include
Exclude
Tissue Histology:
Exclude Cell Lines:
Library Name:

4. Submit query
XXXXXor
5. Reset form

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If you have any questions, comments, or need information about CGAP, please contact the NCI CGAP Help Desk.