New technologies are being developed to miniaturize DNA testing instruments and to improve automation of the processes involved. Both miniaturization and automation have the potential to improve the speed and to reduce the cost of DNA analysis. In addition, miniaturizing the instrumentation required to perform DNA testing could enable examination of biological samples at the crime scene if desired in the future here in the United States.
Current Projects
Separation of Sperm and Epithelial Cells in a Microfluidic Device: An Automated Method for High Efficiency, High Purity Separations
University of Virginia
Award Number: 2006-DN-BX-K021
A SNP-Based Microarray Technology for Use in Forensic Applications
Affymetrix
Award Number: 2005-DA-BX-K101
A Hand-Held DNA-Based Forensic Tool
Cornell University
Award Number: 2004-DN-BX-K001
Nanotechnology DNA Sequencing: Improving DNA Processing Technologies
Brown University
Award Number: 2004-LT-BX-K001
Microfabricated Capillary Array Electrophoresis Genetic Analyzers for Forensic Short Tandem Repeat DNA Profiling
Regents of the University of California
Award Number: 2004-DN-BX-K216
Prototype Microfluidic DNA Analysis System for Forensic Applications
National Institute of Standards and Technology
Award Number: 2003-IJ-R-029
Recently closed
Electro-elution Assisted Purification of DNA for Forensic AnalysisUniversity of Virginia
Award Number: 2005-DA-BX-K100
Final Report (pdf)
Application of a Single Robotics Platform to the Combined Extraction, Purification, Quantification, and Amplification of Forensic DNA Samples
North Louisiana Criminalistics Laboratory
Award Number: 2002-IJ-CX-K019
A Chip-based Genetic Detector for Rapid Identification of Individuals
Nanogen, Inc.
1997-LB-VX-0004
Final Report (pdf)
Microdevice for Automated, Ultra-High Speed, and Portable DNA Forensics
Whitehead Institute
1998-LB-VX-K022
Rapid and high-throughput forensic short tandem repeat typing using a 96-lane microfabricated capillary array electrophoresis microdevice.
Yeung SH, Greenspoon SA, McGuckian A, Crouse CA, Emrich CA, Ban J, Mathies RA.
J Forensic Sci. 2006 Jul;51(4):740-7.
Pub Med
View Pub Med Entry
Grant number: 2004-DN-BX-K216
Microdevice DNA forensics by the simple tandem repeat method.
Goedecke N, McKenna B, El-Difrawy S, Gismondi E, Swenson A, Carey L, Matsudaira P, Ehrlich DJ.
J Chromatogr A. 2006 Apr 14;1111(2):206-13.
Pub Med
View Pub Med Entry
Grant number: 1998-LB-VX-K022
Yeung SH, Greenspoon SA, McGuckian A, Crouse CA, Emrich CA, Ban J, Mathies RA.
J Forensic Sci. 2006 Jul;51(4):740-7.
Pub Med
View Pub Med Entry
Grant number: 2004-DN-BX-K216
Goedecke N, McKenna B, El-Difrawy S, Gismondi E, Swenson A, Carey L, Matsudaira P, Ehrlich DJ.
J Chromatogr A. 2006 Apr 14;1111(2):206-13.
Pub Med
View Pub Med Entry
Grant number: 1998-LB-VX-K022
A high-performance multilane microdevice system designed for the DNA forensics laboratory.
Goedecke N, McKenna B, El-Difrawy S, Carey L, Matsudaira P, Ehrlich D.
Goedecke N, McKenna B, El-Difrawy S, Carey L, Matsudaira P, Ehrlich D.
Electrophoresis. 2004 Jun;25(10-11):1678-86.
Grant number: 1998-LB-VX-K022
Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS.
Vallone PM, Fahr K, Kostrzewa M.
Methods Mol Biol. 2004;297:169-78.
Grant number: 1999-IJ-R-A094
Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis.
Butler JM, Buel E, Crivellente F, McCord BR.
Butler JM, Buel E, Crivellente F, McCord BR.
Electrophoresis. 2004 Jun;25(10-11):1397-412.
Grant number: 1999-IJ-R-A094
Multiple sample amplification and genotyping integrated on a single electronic microarray.
Huang Y, Shirajian J, Schroder A, Yao Z, Summers T, Hodko D, Sosnowski R.
Electrophoresis. 2004 Oct: 25(18-19): 3106-16
View Pub Med Entry
Grant number: 1997-LB-VX-0004
Grant number: 1997-LB-VX-0004
High-speed analysis of multiplexed short tandem repeats with an electrophoretic microdevice.
Mitnik L, Carey L, Burger R, Desmarais S, Koutny L, Wernet O, Matsudaira P, Ehrlich D.
Mitnik L, Carey L, Burger R, Desmarais S, Koutny L, Wernet O, Matsudaira P, Ehrlich D.
Electrophoresis. 2002 Mar;23(5):719-26.
Grant number: 1998-LB-VX-K022
Rapid, high fidelity analysis of simple sequence repeats on an electronically active DNA microchip.
Radtkey R, Feng L, Muralhidar M, Duhon M, Canter D, DiPierro D, Fallon S, Tu E, McElfresh K, Nerenberg M, Sosnowski R.
Radtkey R, Feng L, Muralhidar M, Duhon M, Canter D, DiPierro D, Fallon S, Tu E, McElfresh K, Nerenberg M, Sosnowski R.
Nucleic Acids Res. 2000 Apr 1;28(7):E17.
Grant number: 1997-LB-VX-0004
Two-color multiplexed analysis of eight short tandem repeat loci with an electrophoretic microdevice.
Schmalzing D, Koutny L, Chisholm D, Adourian A, Matsudaira P, Ehrlich D.
Schmalzing D, Koutny L, Chisholm D, Adourian A, Matsudaira P, Ehrlich D.
Anal Biochem. 1999 May 15;270(1):148-52.
Grant number: 1998-LB-VX-K022
Reliable genotyping of short tandem repeat loci without an allelic ladder using time-of-flight mass spectrometry.
Butler JM, Li J, Shaler TA, Monforte JA, Becker CH.
Butler JM, Li J, Shaler TA, Monforte JA, Becker CH.
Int J Legal Med. 1999;112(1):45-9
Grant number: 1997-LB-VX-0003
Single nucleotide polymorphism determination using primer extension and time-of-flight mass spectrometry.
Li J, Butler JM, Tan Y, Lin H, Royer S, Ohler L, Shaler TA, Hunter JM, Pollart DJ, Monforte JA, Becker CH.
Li J, Butler JM, Tan Y, Lin H, Royer S, Ohler L, Shaler TA, Hunter JM, Pollart DJ, Monforte JA, Becker CH.
Electrophoresis. 1999 Jun;20(6):1258-65.
Grant number: 1997-LB-VX-0003
