The I.M.A.G.E. Consortium

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Sequencing Primers

ABI Dye Primers:

T7 Dye Primer.............................5'-TAATACGACTCACTATAGGG-3'
T3 Dye Primer.............................5'-ATTAACCCTCACTAAAGGGA-3'
SP6 Dye Primer..........................5'-ATTTAGGTGACACTATAG-3'
M13 Reverse Dye Primer........5'-CAGGAAACAGCTATGACC-3'
-21M13 Dye Primer..................5'-TGTAAAACGACGGCCAGT-3'

Promega Dye Primers:

-21M13 universal primer.........5'-TGTAAAACGACGGCCAGT-3'
pUC/M13 reverse primer.........5'-CAGGAAACAGCTATGAC-3'
pUC/M13 Forward(-40...........5'-GTTTTCCCAGTCACGAC-3'

Amersham DYEnamic ET primers:

-40 M13 forward.......................5'-GTTTTCCCAGTCACGACG-3'
-21 M13 forward.......................5'-S+SSSSSTGTAAAACGACGGCCAGT-3'
-28 M13 reverse........................5'-AGGAAACAGCTATGACCAT-3'
-28 M13 reverse 2....................5'-AGGAAACAGCTATGACATG-3'
-29 M13 reverse.......................5'-SSSSSST^CAGGAAACAGCTATGACC-3'
SP6.................................................5'-ATTTAGGTGACACTATAG-3'
T7...................................................5'-TAATACGACTCACTATAGGG-3'
T3...................................................5'-ATTAACCCTCACTAAAGGGA-3'
PolyT(N)......................................5'-TTTTTTTTTTTTTTTTTTTTTTT(a,c,g)-3'

Custom primers from Invitrogen:

-21UP...........................................5'-TGTAAACGACGGCCACT-3'
-40UP...........................................5'-GTTTTCCCAGTCACGAC-3'
-40RP...........................................5'-GGATAACAATTTCACACAGG-3'
-100UP.........................................5'-CGCTATTACGCCAGCTGGCGA-3'
-100RP..........................................5'-ATGCAGCTGGCACGACAGGT-3'

Sugano mouse custom primers:

5' end sequencing primer..........5'-CTTCTGCTCTAAAAGCTGCG-3'
3' end sequencing primer..........5'-CGACCTGCAGCTCGAGCACA-3'

University of Tokyo Fugu custom primer:

5' end sequencing primer..........5'-CGCTCTAGAACTAGTGGATCCA-3'

University of Iowa pYX custom primers:

pYX-3 (3' end)..................................5'-ACTCATACTCTTCCTTTTTCAAT-3'
pYX-7 (5' end).................................5'-TTGCTCACATGTTAATACGAC-3'

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